Description
Cap AG (3' Cy5) is a Cy5 labeled cap1 analog which can track mRNA dynamics in live cells.
US patent approved.
Components
|
Cat No. |
Name |
Size |
|
HN3016 |
Cap AG (3' Cy5) (25 mM) |
50 μL |
|
100 μL |
Product Details
|
Molecular Formula |
C66H84N18O30P4S2 (Free acid) |
|
Molecular weight |
1797.51(Free acid) |
|
Concentration |
25 mM |
|
Purity |
HPLC ≥90% |
Figure
Figure 1. Cap Cy5-labeled mRNA can be used for intracellular localization analysis. We applied SM102 LNP to deliver Cy5-LZCap capped mRNA into Huh7 cells, the labeled mRNAs aggregated in the cell which could be readily visualized by confocal microscopy, and over time, Cy5-labeled mRNA gradually co-localized with lysosomes
Figure 2. Cap Cy5-labeled mRNA was used for cell delivery efficiency test in 293T cells. Quantitative results from flow cytometry showed the percentages of Cy5 specific 293T cells at 1h (A) and 4h (B) post transfection compared to control cells.
Storage
This product could be stored at -25~-15 ℃ for two years.
Precautions
- For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
- For research use only