Description
2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff™ HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff™ HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments.
Features
- Convenient, ready-to-use mix
- Thermostable—half-life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
Applications
- Mouse genotyping
- Hotstart PCR
Specifications
| Fidelity (vs. Taq) | 1 × |
| Hot Start | No |
| Overhang | 3'-A |
| Polymerase | Taq DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix (2×) |
Components
| Name | 10108ES03 | 10108ES08 | 10108ES50 | 10108ES60 |
| 2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye) | 1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Shipping and Storage
Shipped on ice packs. Store at -20°C with a shelf life of 2 years.
PCR Reaction（50 μL）
|
Components |
Vol. |
Conc. |
|
ddH2O |
to 50 μL |
- |
|
DNA |
/ |
- |
|
Primer F (10 μM) |
2 μL |
0.4 μM |
|
Primer R (10 μM) |
2 μL |
0.4 μM |
|
2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) |
25 μL |
1× |
|
DNA Type |
Input |
|
Genomic DNA |
50 ng-100 ng |
|
Plasmids |
100 pg-20 ng |
|
cDNA |
1-5 μL |
PCR Cycling
|
Temp. |
Time |
Cycles |
|
95°C |
5 min |
1 |
|
95°C |
30 sec |
35 |
|
50-60°C |
30 sec |
|
|
72°C |
30 sec/kb |
|
|
72°C |
10 min |
1 |
[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)