Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'-3' direction and requires the presence of templates and primers, and T4 DNA Polymerase has 3'→5' exonuclease activity, but does not have 5'→3' exonuclease activity.
Product Components
|
Component number |
Components |
Cat#/Size |
||
|
14451ES03 |
14451ES08 |
14451ES25 |
||
|
14451-A |
T4 DNA Polymerase (25 U/μL) |
1 mL |
5 mL |
25 mL |
Unit definitions
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into polynucleotides in 30 minutes at 37°C.
Product application
T4 DNA Polymerase can be used as followed:
1.Catalyze the blunt ends of the 5' or 3' protruding ends of DNA.
2.Labeled DNA probe synthesis by displacement reaction.
3.Synthesis of the second chain during fixed-point mutation.
4.Cloning of PCR products that do not depend on ligation reactions.
Shipping and Storage
The product is shipped with dry ice and can be stored at -20°C for two years.
Cautions
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!