Description
RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity.
Features
- High Sensitivity：50 copies/T, 25 μL system;
- High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times;
- Specificity: 48-well NTC has no peaks at all, and the specificity is strong;
Applications
- Rapid detection of pathogenic bacteria
- Rapid detection of pet pathogens
- On-site detection of animal husbandry
Specifications
| Detection method | Fluorescent dye method |
| PCR method | RT-LAMP |
| Polymerase | Hieff™ Bst Plus DNA Polymerase |
| Type of sample | RNA |
| LOD | 50 copies/T |
| Specificity | 100% |
Components
| Components No. | Name | 13762ES60 (100 T) | 13762ES80 (1000 T) |
| 13762-A | Bst Buffer | 600 µL | 6 mL |
| 13762-B | Bst Enzyme Mix（UDG plus） | 100 µL | 1 mL |
Storage
Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.
Part 2: 13762-B can be stored at -20°C for 6 months.
Figures
- High Sensitivity
Figure 1: Sensitivity Test
Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.
- Stability
Figure 2.Stability testing at the same temperature
Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test
Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times
- Specificity
Figure 4. 48-well NTC-specific assay
48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.