Description
PCR Enhancer is a mixed additive composed of multiple components. In PCR reaction, PCR Enhancer can effectively reduce the melting temperature of high GC templates and templates with complex secondary structure, and greatly reduce the influence of DNA secondary structure on PCR, which increases the sensitivity and specificity of PCR reaction, and is compatible with almost all DNA polymerase amplification systems. When the optimized PCR program cannot effectively amplify the target fragment, adding PCR Enhancer can often get excellent results. This product may reduce PCR fidelity. Therefore, use it with caution when performing high-fidelity PCR.
Application
Using the human genome as a template, a fragment with a GC content of 72% was amplified using Taq DNA Polymerase.
Quality Control
Exonuclease residue detection: 10 μL of this product and 1 μg of λDNA-Hind III were incubated at 37°C for 4 h, and the electrophoretic bands of DNA did not change.
Endonuclease residues detection: 10 μL of this product and 1 μg of λDNA were incubated at 37°C for 4 h, and the electrophoretic bands of DNA did not change.
Protocols
This product is at a 5× concentration, and the recommended final concentration is 1×. For example, in a 50 μL reaction system, add 10 μL PCR Enhancer.
Figure
Figure 1. Amplification of a gene being with GC content of 72% and length of 690bp using Taq DNA Polymerase (Cat: 10102).
Shipping and Storage
The product is shipped with ice packs and can be stored at -20°C for 2 years.
Cautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. This product is for research use ONLY!