Description
Hifair™ V Reverse Transcriptase is an updated version of HieffTM M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than HieffTM M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. HifairTM V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.
Features
- High-temperature resistance: Suitable for RNA templates with complex secondary structures.
- Suitable for reverse transcription of a small number of templates and low-copy genes.
Applications
- Quantification of fluorescence
- Cloning of genes
Specifications
| Size (Final Product) | 10kb |
| Optimum reaction temperature | 42℃ |
| PCR method | RT-PCR |
| Reverse Transcriptase | M-MLV |
| Type of sample | RNA |
| Ribonuclease H activity | Low activity |
| Product type | cDNA |
Components
| Components No. | Name | 11300ES92 (10000U) | 11300ES93 (5×10000U) |
| 11300-A | Hifair™ V Reverse Transcriptase (200U/μL) | 50 μL | 5×50 μL |
| 11300-B | 5×Hifair™ V Buffer | 250 μL | 5×250 μL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years.
Documents:
Manuals