Description
CapN is a cap analogue with the structure of m7G(5')vppp(5')(2'OMeA)pG , the molecular formula of it is C33H43N15O23P4 and the molecular weight is 1141.68. The product is used for transcription with the initial sequence of 5'AG3', and the natural cap1 structure is produced by Cap1 transcription capping. Compared with Cap0 produced by traditional capping method, the cap1 structure produced by CapN enables the mRNA to have higher activity and translation efficiency in vivo.
Feature
Compared to existing products like CleanCap, this product is an ethylene phosphonic acid-modified mRNA cap analogue. Its double bond structure enhances molecular stability, making it resistant to recognition and hydrolysis by nucleases. With the ethylene group, it effectively stabilizes the structure of the 5' end cap, enhancing the binding ability of mRNA with the eukaryotic initiation factor (eIF4E) and thus improving mRNA translation efficiency. Additionally, this type of cap analogue demonstrates promising data in terms of in vitro transcription yield and capping efficiency.
Components
|
Components No. |
Name |
Cat. No. |
Size |
|
10683 |
CapN (Cap Analog) (100mM) |
10683ES10 |
10 μL |
|
10683ES60 |
100 μL |
||
|
10683ES80 |
1 mL |
Product Details
|
Molecular Formula |
C33H43N15O23P4 (Ammonium Solution) |
|
Molecular weight |
1141.68 |
|
Concentration |
100± 3 mM |
|
Purity |
>98% |
Data
| Cap analogs | mRNA Yield (μg) | mRNA integrity | Capping Rate |
| Cap AG | 182 | 90.50% | 99.00% |
| CapN | 184 | 90.07% | 98.90% |
Table 1. The CapN has similar mRNA yields, integrity and capping rate compared to that of Cap AG.
Storage
This product could be stored at -25~-15 ℃ for two years.
Precautions
- For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
- For research use only.