Description
2×Hieff™ PCR Master Mix is a kind of conventional PCR premixed solution which is ready to use, including Hieff™ Taq DNA Polymerase (Cat#10101), dNTP mix, MgCl2 and optimized buffer. During the reaction, only the primer and template can be added for amplification, which greatly simplifies the operation steps of experiment.
This product contains excellent stabilizers and can be stored for 3 months at 4°C. The PCR product have 3 '-dA protrusion and can be easily cloned into T vector.
Features
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
Applications
- Routine PCR amplification of DNA fragments
- High throughput PCR
Specifications
| Fidelity (vs. Taq) | 1 × |
| Hot Start | No |
| Overhang | 3'-A |
| Polymerase | Taq DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix (2×) |
Components
| Components No. | Name | 10103ES03 | 10103ES08 | 10103ES60 |
| 10103 | 2×Hieff™ PCR Master Mix (With Dye) | 1 mL | 5×1 mL | 100×1 mL |
Storage
The 2×Hieff™ PCR Master Mix products should be stored at -25°C~-15℃ for 2 years.
Figures
2×Hieff™ PCR master mix amplification result
Figure 1. The expected 1.2 kb PCR products can be amplified with 2×Hieff™ PCR Master Mix.
The Master Mix was stored at-20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.
[1] Liu Y, Lai M, Li S, et al. LS-106, a novel EGFR inhibitor targeting C797S, exhibits antitumor activities both in vitro and in vivo. Cancer Sci. 2022;113(2):709-720. doi:10.1111/cas.15229(IF:6.716)