Description
ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit contains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc.
Features
- TFast and accurate ssDNA quantification
- High sensitivity, there is a good linear relationship in the low-concentration range
- Very suitable for the MGI platform library
Applications
- ssDNA quantification
- MGI Circular library and DNB quantification
- BS product quantification
Specifications
| Assay | ssDNA Quantitation |
| Excitation/Emission | 500/525 |
| For Use With (Equipment) | Qubit Fluorometer，fluorescence microplate |
| No. of Reactions | 100T/500T |
| Product Line | Qubit Quantitation |
| Quantitation Range | 1-200 ng, 50pg/μL - 200ng/μL |
| Sample Volume | 1 μL to 20 μL |
| Detection Method | Fluorescent |
Components
| Name | Concentration | 12646ES60 (100T) | 12646ES60 (500T) |
| ssDNA Reagent | 200× in DMSO | 250 μL | 1250 μL |
| ssDNA Buffer | Not applicable | 50 mL | 250 mL |
| ssDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
| ssDNA Standard 2 | 20 ng/μL in TE buffer | 1 mL | 5×1 mL |
Shipping and Storage
All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing.
Figures
Figure 1 Linearity Performance
Test Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng
Figure 2 Comparison of measured values of different batches and original Thermophile products
The stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212)