Murine RNase inhibitor GMP-grade

RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments (such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.

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Product Description

RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase.

This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.).

This product is produced in accordance with GMP process requirements and is provided in liquid form.

Product Properties

Source

Recombinant E.coli with Murine RNase inhibitor gene

Storage Buffer

20 mM Hepes.KOH, 150 mM KCl, 8 mM DTT, 0.5%(v/v)NP 40, 0.5%(v/v)Tween20, 50%(v/v)Glycerol, pH 7.5 ±0.2(25℃)

Unit Definition

The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit

Contents

Catalog No.

Name

Catalog No./Specification

10621ES10

(10 KU)

10621ES20

(20 KU)

10621ES60

(100 KU)

10621ES99

(1 MU)

10621

Murine RNase inhibitor GMP-grade (40 U/μL)

250 μL

500 μL

2.5 mL

25 mL

Shipping and Storage

The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Note

1. This product inhibits RNase activity in a wide range of pH, and exhibits maximum activity at pH 7-8.

2. Avoid foaming or vigorous stirring, vortexing and other operations to prevent the product from deactivation;

3. The product does not inhibit the activity of RNase H;

4. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.